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quinic acid 【藥學】金雞納酸;【化學】奎尼酸。

quinidine

Quinic acid , used shikimate pathway in e . coli , it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell . double specific enzyme genes arog , qutb or three ones arog , qutb , arob were co - expressed in a single plasmid pbv220 to improve the enzymes “ rate - limiting reactions . modifications of e . coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch 利用大腸桿菌莽草酸途徑合成新的代謝物奎尼酸,須在宿主細胞引入異源酶基因擴展代謝途徑;串聯表達酶基因,同時適量增加不同種屬的多個關鍵酶酶量,改善限速反應;利用同源重組進行基因整合和基因破壞,改造染色體結構定向改變微生物代謝途徑;目的是將碳代謝流最大程度的引向奎尼酸生成的方向。